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Specific activity of liver FABP (L-FABP; ng/mg cytosolic protein) increased kelley with length of incubation. An initial peak was observed on day 22, followed by a decline (day 25) and subsequent increase through hatching (day 28) and early posthatch growth. FABP concentrations in the intestine and yolk kelley sac membrane also were measured with the immunoblot and ligand binding assays. The chick liver antisera did not cross-react with yolk sac membrane FABP (YS-FABP), and detectable concentrations of FABP in the intestine (I-FABP) were not observed until hatch (day 28). The ligand binding kelley assay confirmed that I-FABP concentration was very low prior to hatch and increased sharply thereafter, probably in response to dietary fatty acids after hatching. YS-FABP activity as determined by ligand binding assay (dpm/mg yolk sac membrane cytosolic protein) increased from day 13 of incubation to a plateau between days 16 and 19, followed by a decline through hatch. Concentrations in the yolk sac membrane correlated well with the period of maximum lipid transfer from the yolk to the developing embryo via the yolk sac membrane.
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