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Fatty acid methyl esters (FAME) were formed by alkaline hydrolysis of the triacylglycerol and phospholipid fractions followed by treatment of the free fatty acids with methanolic HCl. The FAME derivatives of fatty judith stern acids were separated by capillary gas chromatography (CGC) and analyzed using splitless injection at 200°C, a Supelco SP-2380 capillary column (30m x 0.25mm id.) judith stern in a Hewlett-Packard Model 5890 GC. The quantities of FAME were determined by converting integrated peak area data from the FID response using nonlinear regression slope data for increasing amounts (0.78-200 ng) of the authentic judith stern methyl esters of 14:0, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3 and 20:0 fatty acids. All lipid classes contained variable distributions of 8 fatty acids: the saturated fatty acids, myristic acid (14:0), palmitic acid (16:0), stearic acid (18:0), arachidonic acid (20:0); the monounsaturated fatty acids, palmitoleic acid (16:1), oleic acid (18:1); the polyunsaturated fatty acids, linoleic acid (18:2), linolenic acid (18:3).
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