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| js, feedlot f.l, cryptosporidium, plasma alpha, plump boobs , fruits, fatty oils , prevention, queen fat bottom girls , intestinal fabp, fatty fatty two by four , sterile pbs, plump jack squaw valley , interferon, | Injection of labeled pantothenic acid into the uropygial gland resulted in the synthesis of labeled fatty acid synthetase in which the label appeared to be located exclusively in fatty acid definition the 4'-phosphopantotheine moiety. Analysis of the labeled enzyme by gel filtration and polyacrylamide disc gel electrophoresis in the presence of sodium dodecyl sulfate showed that the labeled pantothenate was fatty acid definition contained exclusively in the half molecular weight moiety. The enzyme contained one 4'-phosphopantetheine residue per subunit (269 000), as determined by measurement of the taurine generated by hydrolysis fatty acid definition of performic acid-treated enzyme. Sodium dodecyl sulfate-activated proteolytic activity was shown to be associated with goose fatty acid synthetase, and this proteolysis was shown to result in the formation of small-molecular-weight protein fragments (less than 200 000) during treatment of the enzyme with sodium dodecyl sulfate. |
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| X 10(-5) M and 1.5 X 10(-5) M were calculated for malonyl-C0A and NADPH, respectively. The fruits major products synthesized by the enzyme from fruits malonyl-CoA and methylmalonyl-C0A were free hexadecanoic acid and free 2, 4, 6, 8-tetramethyldecanoic acid, respectively, with acetyl-CoA as primer. A molecular weight value of 547 000 was determined for the goose fatty acid synthetase by sedimentation equilibrium centrifugation. The purified enzyme had an s20,w of 13.5S and was partially dissociated in low-ionic strength buffer into a 9.3S species, and this dissociation was accompanied by a corresponding partial inactivation of the enzymatic activity. Reassociation and reactivation fruits of the partially dissociated fatty acid synthetase were accomplished in either 0.2 M KCl or 200 muM NADPH. These properties of the goose enzyme are similar to those of other animal fatty acid synthetases, as was the amino acid composition. Dissociation of the purified enzyme with sodium dodecyl sulfate resulted in only two equal molecular weight polypeptides (269 000), as determined by sodium dodecyl sulfate polyacrylamide disc gel electrophoresis. |
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