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What is the role of the troponin complex in enabling cardiac function in fishes from different thermal environments? The troponin (Tn) complex is one of the primary determinants of cardiac calcium omega3s (Ca2+) sensitivity, which in turn controls the rate and strength of cardiac contraction. The goal of this study is to characterize the function of the Tn complex omega3s from different teleost fish species that live at significantly different environmental temperatures and then determine how these alter the functional properties of cardiac muscle. These studies involve i) the expression and purification of recombinant proteins, ii) the use of steady-state omega3s Ca2+ binding assays and stopped-flow kinetic analysis to characterize functional characteristics, and iii) the use of whole troponin exchange to replace native Tn in skinned cardiac trabeculae with recombinant Tns.
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