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| healthy, ozuakee, young fat girls , pleasingly plump , nys department of health, engineering, plump tgp , hey fatty boom boom , fatresistance diet, thermogenics, | These experiments were repeated using another 3 mice from each treatment group, essential fatty acid so that analysis of hepatocytes took place from six ob/ob mice in each feeding group.Hepatic steatosis essential fatty acid was decreased significantly only in the metformin-treated group. The authors found that metformin's beneficial effect on the fatty liver disease of mice was not due to its ability to constrain hyperphagia, nor due to decreased caloric ingestion, because the daily caloric intakes of the metformin-treated mice and the essential fatty acid pair-fed control mice were virtually identical. These caloric intakes were consistently approximately 20% less than that of another obese control group that was permitted to consume diet ad libitum. |
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| Obese ob/ob mice in group 3 (n = 4) and lean mice received no metformin, as with the healthy mice in group 2, but were permitted to consume the liquid diet ad libitum. Liquid diet was given to facilitate accurate daily comparison of food intake among the various treatment groups. All healthy mice were weighed at healthy the beginning of the study and weekly thereafter until killed and then sera, fat, and liver tissues were collected. Tissues were either fixed in buffered formalin and processed from the deceased mice for histology or snap frozen in liquid nitrogen and stored until RNA and proteins were isolated.The feeding protocol was repeated with a second group of 18 ob/ob mice. After 4 wk, hepatocytes were obtained by in situ liver perfusion with collagenase and assayed for cellular adenosine triphosphate (ATP) content. In each experiment, hepatocytes isolated from 3 mice from each treatment group were suspended in a medium and pooled for subsequent analysis to evaluate cell viability, determine the number of obtained cells, and to assay cellular ATP content. |
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