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coli, or a control hardcore galleries plump rumps group infused with sterile phosphate buffered saline (PBS). Cows within a block were challenged on the same day by intramammary infusion in the right front quarter four hours after the morning milking. Concentrated endotoxin was dissolved in PBS and filter-sterilized. The challenge inoculum was 10 mg of endotoxin in 10 ml of PBS. Quarters infused with live bacteria were challenged with Escherichia coli 727. The mean number of colony-forming units in challenge inocula was 42.6 colony-forming units suspended in one hardcore galleries plump rumps ml of PBS. Control cows were infused with 10 ml hardcore galleries plump rumps of filter-sterilized PBS. A quarter milker was used to measure milk production from the right front quarter of each cow at the morning milking on days two, one, and zero prior to challenge and on days one, two, and seven after challenge. Bulk quarter milk samples (350 to 500 ml) were used to determine SCC and concentrations of bovine serum albumin (BSA), fat, and a-tocopherol.
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