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Contribution to a-tocopherol in milk from milk neutrophils was approximately 25% in quarters challenged on days one and two. a-Tocopherol in milk from neutrophils for quarters infused with PBS was 12% on day dha one, 7% on day two, and 8% on day seven after challenge. Discussion Intramammary challenge with E. coli or endo-toxin increased concentrations of a-tocopherol in milk during the acute phase of mastitis but had no effect on concentrations of a-tocopherol in plasma. Concentrations dha of a-tocopherol in milk were 67% higher in quarters challenged with E. coli dha or endotoxin compared with quarters infused with PBS on day two after challenge. In addition, concentrations of a-tocopherol in milk increased more than 80% in quarters challenged with E. coli and 76% in quarters challenged with endotoxin on day two after challenge compared with the pre-challenge concentrations of a-tocopherol in milk.
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