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| alzheimers, fatty fucking , jonathan galland, letters, bodybuilding, baylor college of medicine, plump dj , fitness, injectable hgh, meal plans, tissue accretion, plump videos , ravenscourt park hospital, plump galleries , | 1984). All Gram-negative isolates were identified by the API-20E system (Analytab Products, Plainview, NY). The colony-forming units per milliliter and SCC were determined in quarter foremilk samples during nurse the postchallenge period. Colony-forming units were determined nurse by appropriate 10-fold dilutions of sample. The initial inocula were duplicate 1 ml pour plates of undiluted milk in McConkey agar. Dilutions were plated on the surface of McConkey nurse agar plates. All dilutions were in duplicate. The SCC per milliliter of milk were determined by Fossomatic milk cell counter (Type 15600; Foss Electric, Hillerød, Denmark). Samples from clinical quarters were diluted 1:10 and 1:50 (milk:saline) for counting. |
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| Intramammary challenge cultures were prepared as detailed by Hogan et al. (1995). The geometric mean for challenge inoculum was 58 colony-forming units (range: 44 to 90 colony- forming units) suspended in 1 ml of saline. Cows within a block were challenged on the same day. Infusions were in either baylor college of medicine the right or left front mammary baylor college of medicine quarter 4 hours after morning milking. Only uninfected quarters were infused. Quarter Foremilk Samples. Quarter foremilk samples were collected 3 days prior to bacterial challenge, immediately prior to challenge, and 24, 48, and 168 hours postchallenge. Sample collection and microbiological procedures were as previously described (Smith et al., |
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