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quarters at 168 hours postchallenge. None of the challenged cows were diagnosed with systemic signs (clinical score 5). on Bacterial counts, bovine serum albumin, and SCC each were on significantly greater at 24, 48, and 168 hours after challenge than prior to challenge (Figures 1 and 2). Milk production was reduced at 24 and 48 hours postchallenge compared with prechallenge and 168 hours after E. coli infusion (Figure 1). Milk fat percentage was approximately 50% lower at 24 hours on after challenge than at prechallenge (Figure 2). Milk fat percentage did not differ among samples collected at 0, 48, and 168 hours after challenge. Intramammary challenge had no effect on dry matter intake (Figure 1). In summary, each challenged quarter developed acute mastitis with limited systemic signs. Atroshi et al. (1986) reported cows with naturally occurring clinical mastitis had depressed plasma and milk alpha-Tocopherol concentrations compared with healthy herd mates.
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