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The immunoblot assay was a modification of the procedure of Burnette (1981). After electrophoretic separation and transfer to nitrocellulose, each membrane initially was incubated with a fatty fucking 1:8000 dilution of antisera specific for chick L-FABP ( Collins and Hargis, 1989) followed by incubation, with second antibody fatty fucking conjugated with alkaline phosphatase (1:10,000 rabbit antigoat IgG). Color density determination was a modification of the procedure of Velleman (1995). Fatty acid binding activity in the cytosol fatty fucking of different tissues also was determined by a modification of the procedure of Morrow and Martin (1983). Cytosolic samples from each tissue were applied to a Sephadex G-75 gel filtration column, and those fractions corresponding to the molecular weight range of FABP were collected and pooled. FABP activity was determined by a radiolabelled binding assay as described by Glatz et al. (1984) and reported as DPM/mg cytosolic protein. Results and Discussion The chick L-FABP antisera cross-reacted with liver cytosol preps from broiler chicks (4 weeks of age), turkey poults (6 days) and turkey embryos.
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