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plump girl , plump granny , addicts, nurse, alfalfa silage, on, healthcare, study, docosahexaenoic acid, game, investigation, antiaging, fatty acid products, parenteral vitamin e, raw milk, arts, FABP specific activity (ng/mg protein) increased approximately 40% between hatch and 3 days of age, but total intestinal activity (ng/intestine) increased 10-fold. This is most likely a response jonathan galland to the presence of dietary fatty acids and the involvement of FABP in fatty acid absorptive processes. A major thrust jonathan galland of our research is to try to understand better the mechanisms involved in embryonic transfer from the yolk to the embryo. It was interesting that there was no cross-reactivity between chick L-FABP antisera and yolk sac membrane cytosol. The ligand binding assay, however, showed jonathan galland that FABP specific activity (DPM/mg protein) peaked between 16 and 19 days of incubation, whereas total yolk sac membrane activity was high between 19 days and hatch (day 28). This is the period during which yolk lipids are extensively mobilized and transferred from the yolk to the developing embryo (Ding and Lilburn, 1996).
In a comparison with protein standards, turkey liver FABP has an approximate molecular weight of 14 kD. The concentration of L-FABP increased throughout embryonic development and after hatch. The observed increases during the later stages of incubation correlate well plump granny with documented increases in liver lipid and the movement of fatty acids between the yolk and plump granny the embryo (Ding et al., 1995; Ding and Lilburn, 1996). The increased concentration from hatch through 6 days posthatch may be reflective plump granny of the major physiologic fluxes that occur in liver lipid metabolism during this age period. The chick L-FABP antisera cross-reacted with intestinal FABP and allowed for immunoblot quantitation of I-FABP. Both the immunoblot and ligand binding assay showed that prior to hatch (28 days), there is little FABP in the intestine.
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