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kD; pI 9.0), while Sewel et al. judith (1989) characterized a different chick cytosolic FABP (14 kD; PI 7.0). Katangole and March (1979) reported I-FABP activity in chick intestine during the first week of life but found it to be low compared judith with later ages. There have been no reports to date on FABP activity in liver or intestine from avian judith embryos. The largest proportion of yolk lipids mobilized from the yolk sac to the embryo occurs during the last week of incubation (Ding et al., 1995; Ding and Lilburn, 1996). While there has been much published relative to the contributions of the yolk sac membrane to avian embryonic lipid metabolism, the existence of FABP in this tissue has not been studied. Traditional methods for quantifying FABP activity have incorporated either post-column ligand binding assays using radiolabelled fatty acids or some type of immunoblot assay using protein specific antisera.
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