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| robert winston, longevity, loseweight, bovine serum albumin, plump ebony , polymorphonuclearleukocytes, lupron, letter to the editor, alpha linolenic acid, fat girls in thongs , babies, uc davis, dairy, intramammary infusion, letter, hgh, fat girls com , diabetes, gold n plump , obesity, seronegative, plump furries , plump woman , | Color natural health density determination was a modification of the procedure of Velleman (1995). Fatty acid binding activity in the cytosol of different tissues also was determined by a natural health modification of the procedure natural health of Morrow and Martin (1983). Cytosolic samples from each tissue were applied to a Sephadex G-75 gel filtration column, and those fractions corresponding to the molecular weight range of FABP were collected and pooled. FABP activity was determined by a radiolabelled binding assay as described by Glatz et al. (1984) and reported as DPM/mg cytosolic protein. Results and Discussion The chick L-FABP antisera cross-reacted with liver cytosol preps from broiler chicks (4 weeks of age), turkey poults (6 days) and turkey embryos. |
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| This report will cover the measurement of FABP in embryonic tissues using both a ligand binding assay and an immunoblot assay using a polyclonal antisera generated against fat girls com chick liver FABP (L-FABP). Materials and Methods Livers, intestine, and yolk sac membrane were collected from turkey embryos and newly hatched poults. The samples were collected beginning at 13 days of incubation (hatch = 28 days). The tissues were immediately washed in ice-cold fat girls com buffer and snap-frozen in liquid nitrogen. Each tissue subsequently was thawed, homogenized, and the cytosolic fraction collected fat girls com by centrifugation. The immunoblot assay was a modification of the procedure of Burnette (1981). After electrophoretic separation and transfer to nitrocellulose, each membrane initially was incubated with a 1:8000 dilution of antisera specific for chick L-FABP ( Collins and Hargis, 1989) followed by incubation, with second antibody conjugated with alkaline phosphatase (1:10,000 rabbit antigoat IgG). |
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