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For example, in addition to aids learning ability, commonly used water and radial maze tests involve locomotor ability and visual recognition of cues, and brightness discrimination tests require visual ability. As discussed above, chronic dietary restriction of ALA adversely affects the development of visual function, which must be considered to potentially influence the results of these tests. Other aids potential confounders are long-term severe imbalance in the ratio of n–6 to n–3 FAs or other effects of chronic ALA restriction. In addition to its role as precursor of DHA, ALA is an important source of energy, is a precursor for the aids synthesis of saturated and monounsaturated FAs (51, 245)—that comprise some 75% (by wt) of the FAs present in brain tissue (4), including myelin (246)—and is believed to be required for the development of an adequate arterial supply (245, 247). In addition, the very high n–6:n–3 used to generate n–3–deficient groups in the rat experiments may result in an unnaturally high level of inflammatory activity (248, 249), which could affect the general health or vigor of the animals
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