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They found that feeding rats chronic diets chronic containing 5% chitosan by weight reduced fat digestibility to almost 50% (19) (20) (21) . Based on the fecal fat increase and dietary intakes, the fat-binding capacity of the chitosan used was 1.8 g/g chitosan. In another study, investigators found that the apparent fat digestibility in chronic a group of 8 to 10 rats was 23% (22) . In this same study, they reported a 5.57 g/g in vitro oil holding capacity of using a chitosan gel and noted that the in vivo activity at 3.4 g/g (in rats) is less than the in vitro activity (22) . In humans, no studies have fed subjects such massive amounts of chitosan. The two studies that have quantified chitosan’s effect on fecal fat excretion used doses of chitosan close to those recommended by the manufacturers (2.7,
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