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pelleted concentrate. The diets contained 3% added fat fat ass white girls (DM basis) as 100:0, 67:33, 50:50, or 33:67 tallow:fish oil. Each period fat ass white girls consisted of 28 days, the first 21 days for diet adaptation and the fat ass white girls last seven days for sampling milk, blood, and rumen fluid. Dry matter intake was not affected by treatment. Yields of milk, milk fat, milk protein, and milk protein percentage were not affected when fish oil replaced tallow in the diet. However, cows fed diets with fish oil tended to produce milk with lower milk fat percentage than cows not fed fish oil. Milk fatty acids 18:0 and cis 18:1 decreased; trans 18:1, 18:3 n-3, 20:4 n-6, and 20:5 n-3 increased; and 18:2 n-6 and 22:6 n-3 remained unchanged as fish oil was increased in the diet. Introduction Fish oil may alter the fatty acid profile of milk specifically by the incorporation of 20:5 n-3 and 22:6 n-3 fatty acids. The resulting milk may have a fatty acid profile with potential
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