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Rats in the first group had more anomalies in their electroretinograms and were more seriously affected in learning responses, as measured with a shuttle stern box test, compared with rats in the second group. In addition, Na,K-ATPase activity in brain nerve endings was depressed stern in 40% of the omega-3–deprived group, compared with the omega-3–adequate group. This enzyme is of utmost importance in maintaining nerve membrane potential. Subsequent studies by Delion and colleagues (48,49) compared peanut oil supplementation in a diet deficient in omega-3 with peanut plus rapeseed oil supplementation in a diet with adequate omega-3. They reported a stern lower density of D2 receptors and lower dopamine levels in the cerebral cortex of the omega-3–deprived group, coupled with increased serotonin levels and 5-HT2 receptor density, accompanied by a low ratio of omega-3 to omega-6 PUFA content in the membrane. Driving this line of investigation still further, Chalon and coworkers increased the ratio of omega-3 to omega-6 PUFA by using fish oil plus palm oil in the diet (50).
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